"Arabidopsis thaliana" juxtaposes the implications of injury, development and regeneration for plants, humans and cities, with a nod to one of the most popular plant model organisms in biology and genetics.
Written & directed by Toma Peiu & Nicholas DelRose
Narrated by Selima Smith-Dell
Cinematography by Toma Peiu & Luiza Pârvu
Microscope imagery & graphics by Nicholas DelRose
Editing & sound design by Luiza Pârvu Location sound by Toma Peiu Produced by Nicholas DelRose, Toma Peiu, Luiza Pârvu
Research of Dr. Kenneth Birnbaum’s Lab
Shot on location in New York, October 2016
Shot on Panasonic GH4, iPhone 6, 16 mm Kodak Vision 3 film
A Root Films production, for the Symbiosis Film Competition - Imagine Science Film Festival
Arabidopsis thaliana (2016)
Events of a sunny morning
Arabidopsis thaliana Flower Dissection
Arabidopsis thaliana Flower Dissections and Crossing.
A process that takes about 2 months condensed into 15s. This is how we cross plants in the lab. Arabidopsis is primarily self-fertilizing- so the stamens (male reproductive organs that produce pollen) must be dissected away before they mature and pollinate the pistil (female floral organ). After removing the possibility of self-fertilization, a flower from a different plant can be used to dab pollen onto the dissected flower. The resulting seed pods- or siliques- mature and dry. In this case, transformants that carry an inserted gene of interest also express GFP (Green Fluorescent Protein) in their seed coat, allowing for easy selection.
Watching the setting up of Drop-Seq on our lab floor! Check out these beads running through a microfluidic device on the scope. Drop-seq uses barcoded beads to produce individual cell transcript profiles. This allows us to ask questions about gene expression and cell identity. Find more on Drop-Seq here: >>McCarroll Lab: mccarrolllab.com/dropseq >>Macosko et al., Cell, 2015. #sciart #cinemicro #dropseq #microfluidics #biology #science #art #lablife #phd #imaginescience